Test Entry - Detail

Immunofixation Electrophoresis (Urine)

General

Clinical Use:

Immunofixation electrophoresis is usually ordered when the protein electrophoresis test shows the presence of an abnormal protein band that requires typing or confirmation. Urine immunofixation is used to confirm the presence of Bence Jones Protein. Immunofixation electrophoresis consists of two distinct steps in the procedure; the first stage is high-resolution electrophoresis followed in the second stage by application of monospecific antisera to the surface of the gel to precipitate the antigen(s) of interest.
Routinely a detection screen is performed. A detection screen consists of Protein Electrophoresis of Serum (QEP) and/or Protein Electrophoresis of Urine (BJC or BJE). If an abnormal protein band or suspected abnormal band is seen in the QEP an Immunofixation is performed. If an Immunofixation is required quantitation of Immunoglobulins IgG, IgA and IgM will be performed. Immunofixation routinely tests for alpha, gamma, mu, kappa and lambda chains. (D & E can be tested for if required).

Availability:

Samples are tested Monday-Friday.

Aliases/Synonyms:

IEF (Urine), IFE (Urine), IMF (Urine),

Code:

UIFE

Handling Instructions (to laboratory):

Send sample chilled.

Hyperlink:

Reference Interval:

Refer to report or laboratory

Collection Requirements

Container:

Urine Container (URINE),

Sample Type:

See container

Minimum Collection Volume:

Collection Instructions:

Processing Requirements

Alternate Containers:

Processing Instructions:

Minimum Assay Volume:

20mL

Stability:

7 days chilled

Transport Instructions (to testing laboratory):

Send sample chilled.

Testing Locations

Performed at:

Section	Department	Site	Contact Phone
Special Chemistry	Biochemistry	Fiona Stanley Hospital	6152 2830
Special Chemistry	Biochemistry	QEII Medical Centre	6383 4114

Last Updated : 08-10-2020 08:38