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Engraftment Studies (Short Tandem Repeats)
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General
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Clinical Use:
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These PCR's are used to monitor the engraftment status of a patient after BMT. They create a partial DNA fingerprint that indicates the presence of donor and/or recipient DNA, thereby indicating the source of reconstitution of the bone marrow after transplant. Samples from the transplant donor and recipient are required prior to transplant to ascertain the unique pattern for each source of DNA in each loci tested (8 in total). Peripheral blood samples are then collected from the patient post transplant to monitor the engraftment status. The progressive change in DNA from recipient to donor (indicated by the change in the size of the PCR products) is indicative of successful marrow engraftment. Persistent or reappearance of recipient DNA is suggestive of failure to engraft with donor cells or relapse. Persistence of both donor and recipient DNA indicates a mixed chimaeric state. |
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Availability:
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As required. |
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Aliases/Synonyms:
| Chimerism studies (Engraftment), STR, Variable Number of Tandem Repeats, VNTR, |
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Code:
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HESTR |
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Handling Instructions (to laboratory):
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Send sample ambient. |
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Hyperlink:
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Reference Interval:
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Results are reported as percentage of donor present
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Collection Requirements
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Container:
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EDTA (LAVENDER), |
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Sample Type:
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Blood; Bone Marrow; Stem Cells |
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Minimum Collection Volume:
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3mL Blood; 1mL Bone Marrow; 0.1mL Stem Cells |
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Collection Instructions:
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Non-medicare rebateable item. All patients except Public, VA and Pensioners must pay $173.55 upfront.
Collectors, please collect a dedicated EDTA for this test. |
Processing Requirements
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Alternate Containers:
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EDTA (LAV9) |
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Processing Instructions:
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Store sample at room temperature. |
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Minimum Assay Volume:
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Stability:
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96 hours ambient |
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Transport Instructions (to testing laboratory):
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Send sample ambient. |
Testing Locations
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Performed at:
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Section
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Department
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Site
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Contact Phone
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| Molecular |
Haematology |
Fiona Stanley Hospital |
6152 8118 |
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Last Updated : 27-11-2024 11:19 |