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PML-RARA Quantitative Real Time PCR
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General
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Clinical Use:
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These real time quantitative PCR assays for each of the t(15;17) breakpoints (BCR1, 2, 3) allow the exact measurement of a patients' level of disease by calculating the number of copies of t(15;17) (ie PML-RARA fusion gene transcripts). Transcript levels less than 10 are considered below the detection limit of the assay and are called negative but are reported as <10 to indicate that some amplification occurred. Transcript levels that do not amplify at all are denoted a value of zero. A control gene (ABL) must amplify a predetermined number of copies (depending on the patients level of disease) to qualify that the sample is of sufficient quality and quantity to validate the result. A recollect is recommended if the control gene fails and a "no result" issued. Patient % ratio results are most informative when considered serially, observing the change in level of disease over time. Note that up to a 2-fold change in consecutive results is considered normal variation of the assay, a 2-10-fold increase suggestive of impending relapse (retest to confirm and monitor patient more closely) and a >10-fold increase defines molecular relapse. |
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Availability:
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Samples are tested Monday-Friday. |
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Code:
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HPRQN |
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Handling Instructions (to laboratory):
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Send sample ambient. |
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Hyperlink:
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Reference Interval:
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Results are reported as a normalised ratio of the number of copies of PML-RARA transcripts divided by the number copies of the control gene (ABL) detected in a defined volume of cDNA. Results are expressed as a percentage ratio.
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Collection Requirements
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Container:
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EDTA x 2 (LAV9), |
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Sample Type:
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Blood; Bone Marrow. For other specimen types, please contact the laboratory on (08) 6152 8118 to discuss. |
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Minimum Collection Volume:
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Collection Instructions:
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Regional Services: Collect sample Monday-Thursday only.
Do not collect prior to public holidays. |
Processing Requirements
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Alternate Containers:
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Processing Instructions:
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Store sample at room temperature. |
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Minimum Assay Volume:
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3mL (Blood); 1mL (Bone Marrow) |
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Stability:
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48 hours ambient |
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Transport Instructions (to testing laboratory):
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Send sample ambient.
Please contact the testing laboratory to inform them prior to collecting or sending the sample on a Friday. |
Testing Locations
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Performed at:
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Section
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Department
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Site
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Contact Phone
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| Molecular |
Haematology |
Fiona Stanley Hospital |
6152 8118 |
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Last Updated : 15-04-2025 09:46 |