Test Entry - Detail

Tobramycin


General
  Clinical Use: To determine the concentration of tobramycin in the blood in order to maintain a therapeutic level or to detect a toxic level.
  Availability: Samples are tested routinely during the testing laboratory's hours of operation.
 
  Code: TOB
  Handling Instructions (to laboratory): Send sample chilled.
  Hyperlink:
  Reference Interval:
Monitoring of the aminoglycoside plasma concentrations should begin from the first dose if used for directed therapy, or if empiric therapy is planned to extend beyond 48 hours.
Monitoring is essential to maximise efficacy and to prevent toxicity.
For once daily dosing a computerised method approach is recommended. This requires two plasma concentrations (peak and post dose levels)
entered into a computer program to calculate drug exposure. Discuss with a Clinical Pharmacist regarding locally available calculators.
Trough levels should remain below the detectability of the assay.
For multiple-daily, synergistic dosing regimens (8 to 12 hourly) of gentamicin (eg for endocarditis), measure a trough (pre-dose) concentration, aiming for 0.5 to 1mg/L.
For prolonged courses of aminoglycoside therapy (>7 days) or where high cumulative aminoglycoside exposure has occurred, regular clinical and audiologic monitoring
is strongly recommended due to the risk of ototoxicity.
Contact an Infectious Disease Physician, Clinical Microbiologist or Clinical Pharmacist for further advice on dosing and therapeutic monitoring.


Collection Requirements
  Container: Lithium Heparin-PST (GREEN),  
  Sample Type: See container
  Minimum Collection Volume: 300uL
  Collection Instructions: If sample will not reach laboratory within 24 hours of collection, it must be centrifuged and separated from gel. Alternatively a non-gel tube should be used.


Processing Requirements
  Alternate Containers:
Lithium Heparin, No Gel (DKGNLITH)
Serum, no gel (RED)
  Processing Instructions:
  Minimum Assay Volume: 150uL
  Stability: 24 hours chilled (unseparated); 7 days chilled (separated)
  Transport Instructions (to testing laboratory): Send sample chilled.


Testing Locations
  Performed at:
Section Department Site Contact Phone
Core Biochemistry Fiona Stanley Hospital 6152 8140
Core Biochemistry QEII Medical Centre 6383 4093


Last Updated : 14-10-2020 14:47