Test Entry - Detail

t(9;22) BCRABL Quantitative Real-Time PCR

General

Clinical Use:

The real time quantitative PCR assay for the t(9;22) major breakpoints (B3A2, B2A2) allows the measurement of a patient's level of disease by calculating the number of copies of t(9;22) (ie BCRABL fusion gene transcripts). Patient BCR-ABL IS% is most informative when considered serially, observing the change in level of disease over time.

Availability:

Samples are tested Monday-Friday.

Aliases/Synonyms:

BCR-ABL, BCRABL Major Quantitative PCR, BCRABL Minor Quantitative PCR, Molecular Haematology PCR BCQ, Quantitative BCRABL,

Code:

HBCQN (Use this code for ALL BCR Requests)

Handling Instructions (to laboratory):

Send sample ambient.
Sample must reach laboratory within 48 hours of collection.

Hyperlink:

Reference Interval:

Results are reported as a normalised ratio of the number of copies of BCR-ABL divided by the number of copies of the control gene (ABL) detected in a standard volume of blood, expressed as a percentage. Contact the laboratory for additional information.

Collection Requirements

Container:

EDTA x 2 (LAV9),

Sample Type:

Blood; Bone Marrow. For other specimen types, please contact the laboratory on (08) 6152 8118 to discuss.

Minimum Collection Volume:

9mL Blood; 1mL Bone Marrow

Collection Instructions:

Regional Services: Collect sample Monday-Thursday only.
Do not collect prior to public holidays.

Processing Requirements

Alternate Containers:

Processing Instructions:

Store sample at room temperature.
Do not centrifuge sample.

Minimum Assay Volume:

9mL Blood; 1mL Bone Marrow

Stability:

48 hours ambient

Transport Instructions (to testing laboratory):

Send sample ambient.
Please contact the testing laboratory to inform them prior to collecting or sending the sample on a Friday.

Testing Locations

Performed at:

Section	Department	Site	Contact Phone
Molecular	Haematology	Fiona Stanley Hospital	6152 8118

Last Updated : 18-07-2025 13:28