|
CBFB-MYH11 Quantitative PCR
|
|
General
|
| |
Clinical Use:
|
The real time quantitative PCR assay for each of the inversion 16 or t(16;16) breakpoints (Type A and E) allow the exact measurement of a patient's level of disease by calculating the number of copies of inversion 16 or t(16;16) (ie CBFB-MYH11 fusion gene transcripts). Transcript levels less than 10 are considered below the detection limit of the assay and are called negative but are reported as <10 to indicate that some amplification occurred. Transcript levels that do not amplify at all are denoted a value of zero. A control gene (ABL) must amplify a predetermined number of copies (depending on the patients level of disease) to qualify that the sample is of sufficient quality and quantity to validate the result. A recollect is recommended if the control gene fails and a "no result" issued. Patient % ratio results are most informative when considered serially, observing the change in level of disease over time. Note that up to a 2-fold change in consecutive results is considered normal variation of the assay, a 2-10-fold increase suggestive of impending relapse (retest to confirm and monitor patient more closely) and a >10-fold increase defines molecular relapse.
Qualitative PCR can be performed for the other rarer breakpoints (eg. Type E) |
| |
Availability:
|
Samples are tested Monday-Friday. |
| |
Aliases/Synonyms:
| Inversion 16 Quantitative Real Time PCR, t(16;16) Quantitative Real Time PCR, |
| |
Code:
|
HCBQN |
| |
Handling Instructions (to laboratory):
|
Send sample ambient. |
| |
Hyperlink:
|
|
| |
Reference Interval:
|
Results are reported as a normalised ratio of the number of copies of target transcripts divided by the number copies of the control gene (ABL) detected in a defined volume of cDNA. Results are expressed as a percentage ratio.
|
Collection Requirements
|
| |
Container:
|
EDTA x 2 (LAV9), |
| |
Sample Type:
|
Blood; Bone Marrow. For other specimen types, please contact the laboratory on (08) 6152 8118 to discuss. |
| |
Minimum Collection Volume:
|
|
| |
Collection Instructions:
|
Regional Services: Collect sample Monday-Thursday only.
Do not collect prior to public holidays. |
Processing Requirements
|
| |
Alternate Containers:
|
|
| |
Processing Instructions:
|
Store sample at room temperature. |
| |
Minimum Assay Volume:
|
3mL Blood; 1mL Bone Marrow |
| |
Stability:
|
48 hours ambient |
| |
Transport Instructions (to testing laboratory):
|
Send sample ambient.
Please contact the testing laboratory to inform them prior to collecting or sending the sample on a Friday. |
Testing Locations
|
| |
Performed at:
|
|
Section
|
Department
|
Site
|
Contact Phone
|
| Molecular |
Haematology |
Fiona Stanley Hospital |
6152 8118 |
|
Last Updated : 15-04-2025 09:49 |